Biofilm-specific physiologies of staphylococcus aureus.

by Anne Elizabeth Hodgson

Publisher: Universityof Manchester in Manchester

Written in English
Published: Pages: 296 Downloads: 587
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Edition Notes

Manchester thesis (Ph.D.), Department of Pharmacy.

ContributionsUniversity of Manchester. Department of Pharmacy.
The Physical Object
Pagination296p.
Number of Pages296
ID Numbers
Open LibraryOL16572352M

Staphylococcus, (genus Staphylococcus), group of spherical bacteria, the best-known species of which are universally present in great numbers on the mucous membranes and skin of humans and other warm-blooded term staphylococcus, generally used for all the species, refers to the cells’ habit of aggregating in grapelike clusters.. Staphylococci are microbiologically characterized. Staphylococcus aureus, including Methicillin-resistant Staphylococcus aureus, are one of the most common causes of healthcare-associated infections. The first report of Vancomycin Resistant Staphylococcus aureus (VRSA) came in VRSA is also resistant to methicillin and other classes of antibiotics, limiting the available treatment options. In staphylococcus various strains of the species S. aureus and S. epidermidis. While S. epidermidis is a mild pathogen, opportunistic only in people with lowered resistance, strains of S. aureus are major agents of wound infections, boils, and other human skin infections and are one of the most common causes of food Read More. One of the most important traits of pathogenic microbial biofilms is their high tolerance to conventional antimicrobial agents, which is partially due to the presence of metabolically inactive and transiently resistant persister cells. Here, we use guanine-rich DNA structures known as G-quadruplexes (G4s) coupled with the β-lactam antibiotic, oxacillin (OX), and loaded with an iron-containing.

Antibiotic pressure can induce the viable but non-culturable state in Staphylococcus aureus growing in biofilms S. Pasquaroli. 1. Department of Life and Environmental Sciences attributed to factors that include the presence of biofilm-associated extracellular polymeric substances as well as biofilm-specific microbial physiologies. What is claimed is: 1. An immunogenic composition comprising: (a) five purified Staphylococcus aureus polypeptides, or portions thereof, or variants thereof, or combinations thereof, wherein the S. aureus polypeptides are (i) biofilm-specific S. aureus polypeptide SA set forth in SEQ ID NO: 13, (ii) planktonic-specific S. aureus polypeptide SA set forth in SEQ ID NO: 14, (iii) biofilm.   This volume offers a comprehensive overview of basic and applied aspects of Staphylococcus aureus, which is one of the most important human includes sixteen chapters that address the microbiology and immunology of S. aureus, the pathology of its key manifestations, and the current standard of r, it reviews cutting-edge advances in Manufacturer: Springer.   Introduction. Two staphylococcal species, Staphylococcus aureus and Staphylococcus epidermidis, are the main cause of hospital-associated infections,. S. epidermidis is a common etiological agent of nosocomial infections, mostly occurring in immune compromised patients with implanted medical illin-resistant S. aureus (MRSA) strains are also responsible .

  Staphylococcus aureus is now acknowledged as being the most important bacterial pathogen of humans. It usually produces localized disease but can be rapidly invasive, spreading through the tissues, invading bone, and seeding the bloodstream to produce a fulminant picture of septic shock, disseminated intravascular coagulation, and rapid death.   Staphylococcus aureus is a gram-positive, beta hemolytic, catalase and coagulase positive organism that commonly colonizes the nares. It produces toxic shock syndrome toxin, exfoliative toxin, and enterotoxin. These cause toxic shock syndrome, scalded skin syndrome, and rapid-onset food poisoning, respectively. The ability to form persisters has been observed in many microorganisms, including Staphylococcus aureus, mainly in the context of chronic infections and the pathogenicity of these our research, we have demonstrated that salt or oxidative stress could play a role in the formation of S. aureus persisters outside the host's intracellular interface.   Oxacillin Coupled G-Quadruplexes as a Novel Biofilm-Specific Antibiotic for Staphylococcus aureus Biofilms Renzo A. Fenati School of Chemical and Biomedical Engineering, Melbourne School of Engineering, The University of Melbourne, Parkville, Victoria , Australia.

Biofilm-specific physiologies of staphylococcus aureus. by Anne Elizabeth Hodgson Download PDF EPUB FB2

Staphylococcus aureus is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system.S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections.

The formation of a biofilm, and encasement of cells in a polymer-based matrix Cited by:   Model of Staphylococcus aureus biofilm development. aureus biofilm development is described in five stages: (A) attachment, (B) multiplication, (C) exodus, (D) maturation and (E) dispersal.

aureus cells attach to abiotic or biotic surfaces via hydrophobic interactions or MSCRAMMs, respectively. After cells attach, the biofilm develops into a confluent ‘mat’ of cells Cited by: Staphylococcus aureus is a Gram-positive bacterium that typically resides asymptomatically in the anterior nares and the skin of mammals.

Since its discovery in the s, it has been recognized as a major opportunistic pathogen in humans, responsible for various diseases, ranging from minor skin infections to severe bacteremia and necrotizing.

The majority of staphylococci produce biofilm on medical devices, which is the main mechanism to infect humans. Staphylococcal biofilms attach to abiotic or biotic surfaces, forming aggregates and protecting themselves against the immune system and the antimicrobial compounds of the host.

Few studies on biofilm formation mechanism in Staphylococcus epidermidis and other coagulase-negative. The strains of four pathogenic species of the genus Candida (C. albicans, C. glabrata, C. parapsilosis and C.

tropicalis) and of Staphylococcus aureus were employed to determine the effective relatedness among techniques and the specific activity of the biofilm, as a Cited by: 9.

In Staphylococcus aureus, the de novo synthesis of biosynthetic precursors requires thirteen biosynthetic intermediates (e.g., glucosephosphate and oxaloacetate) and the coordinated action of numerous enzymes.

The life-cycle of S. aureus is such that its environment is in a near constant state of flux. This flux has important implications. Staphylococcus aureus often lead to significant increases in morbidity and mortality, particularly when associated with indwelling medical devices.

This has triggered a great deal of research attempting to understand the molecular mechanisms that con-trol S. aureus biofilm formation and the basis for the. The strains of four pathogenic species of the genus Candida (C.

albicans, C. glabrata, C. parapsilosis and C. tropicalis) and of Staphylococcus aureus were employed to determine the effective relatedness among techniques and the specific activity of the biofilm, as a ratio between the XTT and the CV outcomes.

Since the ability to form biomass. This book brings together respected S. aureus experts from around the world to provide a timely overview of staphylococcal research. Essential reading for scientists working with staphylococci. This text is an excellent introduction for entry level scientists, as well as those seeking a deeper understanding of this critically important bacterial pathogen.

Staphylococcus aureus is a major human pathogen that causes a wide range of clinical infections. It is a leading cause of bacteremia and infective endocarditis as well as osteoarticular, skin and soft tissue, pleuropulmonary, and device-related infections.

This review comprehensively covers the epidemiology, pathophysiology, clinical manifestations, and management of each of these clinical. Characteristics of Staphylococcus aureus infections include biofilm formation, leading to the spread of bacteria to the bloodstream causing sepsis and metastatic infections.

In particular, in methicillin-resistant S. aureus (MRSA) infections, biofilm formation critically hampers treatment and causes poor prognosis. We explored the biofilm formation of MRSA in the presence or absence of. Staphylococcus aureus Biofilms Staphylococcus aureus is a Gram-positive, ubiquitous bacterial spe - cies.

The ecological niche of S. aureus in humans is the anterior nares. In the human population, approximately 20–25% have become persistently colonized and 75–80% intermittently or never colonized,16 Previous studies have shown that.

This chapter addresses primarily staphylococcal food poisoning (SFP); however, in regard to the staphylococcal enterotoxins (SEs), there is significant overlap in the natural histories of both diseases. Hence, toxic shock syndrome (TSS) is also discussed in the chapter in which this overlap is most relevant.

The chapter discusses the nomenclature and evolution of the SE family of toxins. Staphylococcus aureus is part of the natural skin flora, preferentially colonizing external mucosa in 30 to 50% of the population, healthy carriers who develop no symptoms.

But it. Purchase Staphylococcus aureus - 1st Edition. Print Book & E-Book. ISBNStaphylococci, with the leading species Staphylococcus aureus and Staphylococcus epidermidis, are the most frequent causes of infections on indwelling medical devices.

The biofilm phenotype that those bacteria adopt during device-associated infection facilitates increased resistance to antibiotics and host immune defenses. This review presents and discusses the molecular mechanisms.

Staphylococcus aureus is a versatile and virulent pathogen in humans, who serve as natural reservoirs for this pathogen [].The rates of infections caused by staphylococci, both community- and hospital-acquired strains, are increasing steadily [2, 3].Concurrently, treatment of these infections is becoming more difficult because of the increasing prevalence of multidrug-resistant strains [].

Staphylococcus aureus is resident in skin and nasal membranes with a formidable pathogenic potential to cause a variety of community and hospital acquired infections. This authoritative survey covers the impact of genome sequences into our understanding of MRSA and MSSA pathogenesis.

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that is a member of the Firmicutes, and it is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the is often positive for catalase and nitrate reduction and is a facultative anaerobe that can grow without the need for oxygen.

Although S. aureus usually acts as a commensal. The Discovery and Naming of Staphylococcus aureus Abigail Orenstein Alexander Ogston () was a Scottish surgeon who in discovered the major cause of pus. Distressed with the high rate of post-operative mortality and unwilling to accept death as a.

Staphylococcus aureus Staphylococcus aureus is a major pathogen of increas-ing importance due to the rise in antibiotic resistance (Lowy, ). It is distinct from the CoNS (e.g.S. epidermidis), and more virulent despite their phylogenic similarities (W aldvogel, ; Projan and Novick, ).

The species named aureus, refers to the fact that. infections, and Staphylococcus aureus is the leading species in this domain [6–8]. Bio lm is de ned as a multicellular lifestyle, an organized structure built by almost all bacte - rial species. Staphylococcus aureus is the most dangerous of all of the many common staphylococcal bacteria.

These gram-positive, sphere-shaped (coccal) bacteria (see figure How Bacteria Shape Up) often cause skin infections but can cause pneumonia, heart valve infections, and bone infections.

Background: The growth of Staphylococcus aureus in foods presents a potential public health hazard since many strains of S. aureus produce enterotoxins that case food poisoning if ingested. Among the reasons for examining foods for S. aureus are. to confirm that this organism may be the causative agent of foodborne illness; to determine whether a food or food ingredient is a potential source.

1 Staphylococcus aureus Staphylococcus aureus is a bacterium that causes staphylococcal food poisoning, a form of gastroenteritis with rapid onset of symptoms.

aureus is commonly found in the environment (soil, water and air) and is also found in the nose and on the skin of humans. Staphylococcus aureus strains are an important medical infectious agent that causes a wide range of pathogeneses starting from colonization of the skin and mucosal surface to severe pathogenic effects such as septicemia.

The mortality and morbidity from this pathogen are challenging issues for the healthcare premises. Methicillin Resistant Staphylococcus aureus strains (MRSA) are. The need for bone and joint prostheses is currently growing due to population aging, leading to an increase in prosthetic joint infection cases.

Biofilms represent an adaptive and quite common bacterial response to several stress factors which confer an important protection to bacteria. Biofilm formation starts with bacterial adhesion on a surface, such as an orthopedic prosthesis, further.

Staphylococcus aureus (S. aureus) is a gram-positive bacterium which has been recognized as a major pathogen infecting a range of hosts [2, 3] and has been employed in.

The life of Staphylococcus aureus bacteria in human. The antibiotic help us kills them. #05/07/ Nguồn: New Era Stem Cell. Keywords: biofilm, Staphylococcus aureus, RTCA, M LOABE, specific antibiofilm activity, phage lytic proteins, exopolysaccharide depolymerase.

Citation: Gutiérrez D, Fernández L, Martínez B, Ruas-Madiedo P, García P and Rodríguez A () Real-Time Assessment of Staphylococcus aureus Biofilm Disruption by Phage-Derived Proteins.

Front. Although more than 20 species of Staphylococcus are described in Bergey's Manual (), only Staphylococcus aureus and Staphylococcus epidermidis are significant in their interactions with humans.

S. aureus colonizes mainly the nasal passages, but it may be found regularly in most other anatomical locales, including the skin, oral cavity and.Out of 50 isolates of Staphylococcus aureus, 33 (%) were MRSA, of which 20(%) were positive for biofilm production.

Also MDR was more frequent in biofilm producers in comparison to non-biofilm producers. DISCUSSION Infections by Staphylococcus aureus are a major problem in hospital settings, especially among patients with indwelling.Figure 2. Distribution percentages of strong, weak and negative biofilm producers of Staphylococcus aureus isolates for different biofilm quantitative methods based on the corresponding calculated.